The Phospho-HSF1-S307 Polyclonal Antibody (CABP1396) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By binding to the phosphorylated form of HSF1, researchers can detect and analyze its activity in various cellular contexts.HSF1, a key regulator of the heat shock response, plays a crucial role in proteostasis and cellular stress response. Phosphorylation at serine 307 is known to modulate HSF1 activity, influencing its ability to regulate the transcription of heat shock proteins.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-HSF1-S307 Polyclonal Antibody
SKU:
CABP1396
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HSTF1, Phospho-HSF1-S307
Positive Sample:
HUVEC
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
57kDa
Observed MW:
76kDa
The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90.
Purification Method
Affinity purification
Gene ID
3297
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Phospho-HSF1-S307 Rabbit pAb (CABP1396) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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