Phospho-Histone H3.3-S31 Antibody (CABP1296)

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SKU:
CABP1296
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Phospho-Histone H3.3-S31 Antibody (CABP1296)
Antibody SKU:CABP1296
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A phospho synthetic peptide corresponding to residues surrounding S31 of Human Histone H3.3.
Application:Western blotting, Immunohistochemistry
Recommended Dilution:WB 1:500 - 1:2000
IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, NIH/3T3, C6
Immunogen:A phospho synthetic peptide corresponding to residues surrounding S31 of Human Histone H3.3.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Cellular Location:Chromosome, Nucleus
Calculated MW:15kDa
Observed MW:15KDa
Synonyms:H3F3B, H3.3B
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17.
Immunohistochemistry of paraffin-embedded rat kidney using Phospho-Histone H3. 3-S31 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded rat kidney using Phospho-Histone H3. 3-S31 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone H3. 3-S31 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone H3. 3-S31 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-Histone H3. 3-S31 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-Histone H3. 3-S31 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human esophageal cancer using Phospho-Histone H3. 3-S31 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human esophageal cancer using Phospho-Histone H3. 3-S31 Rabbit mAb at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H3. 3-S31 antibody at 1:1000 dilution. NIH/3T3 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H3. 3-S31 antibody at 1:1000 dilution. NIH/3T3 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using Phospho-Histone H3. 3-S31 antibody at 1:1000 dilution. HeLa cells and C6 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Western blot analysis of extracts of various cell lines, using Phospho-Histone H3. 3-S31 antibody at 1:1000 dilution. HeLa cells and C6 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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