Phospho-Ets1 (Thr38) Antibody (PACO24525)
- SKU:
- PACO24525
Description
| Antibody Name: | Phospho-Ets1 (Thr38) Antibody (PACO24525) |
| Antibody SKU: | PACO24525 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Peptide sequence around phosphorylation site of threonine 38(L-L-T(p)-P-S) derived from Human ETS1 . |
| Form: | Liquid |
| Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from HepG2 cells using ETS1 (Phospho-Thr38) Antibody.The lane on the right is treated with the antigen-specific peptide. |
 | Immunofluorescence staining of methanol-fixed HeLa cells using ETS1 (Phospho-Thr38) Antibody. |
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using ETS1 (Phospho-Thr38) antibody (left)or the same antibody preincubated with blocking peptide (right). |
| Background: | Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion. |
| Synonyms: | p54; ETS1; ETS-1; C-ets-1 protein; |
| UniProt Protein Function: | Ets-1: a proto-oncogenic transcription factor homologous to the v-ets erythroblastosis virus oncogene. The DNA binding activity of Ets1 is controlled by kinases and transcription factors. It contributes to the regulation of cellular differentiation in hematopoietic cells. Ets1 promotes invasive behavior in endothelial cells, vascular smooth muscle cells and epithelial cancer cells. Regulates the expression of MMP1, MMP3, MMP9 and uPA as well as of VEGF and VEGFR gene expression. Two alternatively spliced isoforms have been described.Protein type: Motility/polarity/chemotaxis; Transcription factor; Oncoprotein; DNA-bindingChromosomal Location of Human Ortholog: 11q23.3Cellular Component: nucleoplasm; transcription factor complex; intercellular bridge; cytoplasm; nucleusMolecular Function: histone acetyltransferase binding; protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activityBiological Process: transcription from RNA polymerase II promoter; hypothalamus development; positive regulation of erythrocyte differentiation; positive regulation of transcription, DNA-dependent; cell motility involved in cell locomotion; PML body organization and biogenesis; female pregnancy; positive regulation of cell motility; angiogenesis involved in wound healing; regulation of angiogenesis; response to estradiol stimulus; negative regulation of cell cycle; regulation of apoptosis; response to antibiotic; negative regulation of cell proliferation; positive regulation of angiogenesis; pituitary gland development; response to mechanical stimulus; positive regulation of cell proliferation; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; immune response |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] |
| UniProt Code: | P27577 |
| NCBI GenInfo Identifier: | 219689118 |
| NCBI Gene ID: | 2113 |
| NCBI Accession: | NP_001137292.1 |
| UniProt Secondary Accession: | P27577,Q14278, Q16080, Q6N087, Q96AC5, A9UL17, F5GYX9 |
| UniProt Related Accession: | P27577,P14921 |
| Molecular Weight: | |
| NCBI Full Name: | protein C-ets-1 isoform 1 |
| NCBI Synonym Full Names: | v-ets avian erythroblastosis virus E26 oncogene homolog 1 |
| NCBI Official Symbol: | ETS1 |
| NCBI Official Synonym Symbols: | p54; ETS-1; EWSR2 |
| NCBI Protein Information: | protein C-ets-1; v-ets avian erythroblastosis virus E2 oncogene homolog 1; Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1 |
| UniProt Protein Name: | Protein C-ets-1 |
| UniProt Synonym Protein Names: | p54 |
| Protein Family: | Protein |
| UniProt Gene Name: | ETS1 |
| UniProt Entry Name: | ETS1_HUMAN |
Additional Information
Product Type: |
Antibody |
Reactivity: |
Human |
Reactivity: |
Mouse |
Host Species: |
Rabbit |
Isotype: |
IgG |
Applications: |
ELISA |
Applications: |
WB |
Applications: |
IHC |
Applications: |
IF |
Antibody Type: |
Polyclonal Antibody |
Conjugation: |
Unconjugated |
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