Immunology Antibodies 3

ETS1 Polyclonal Antibody (CAB1596)

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SKU:
CAB1596
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
Reactivity:
Human, Mouse
Synonyms:
p54, ETS-1, EWSR2, c-ets-1, ETS1
Research Use:
Immunology
Frequently bought together:

Description

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ETS1 Polyclonal Antibody (CAB1596)

The ETS1 Polyclonal Antibody (CAB1596) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has high reactivity with human samples and is validated for use in various applications, including Western blotting. By binding specifically to the ETS1 protein, researchers can detect and analyze its presence in a wide range of cell types, making it a versatile tool for studies in molecular biology and cancer research.

This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:ETS1 Polyclonal Antibody
SKU:CAB1596
Size:20μL, 100μL
Reactivity:Human, Mouse
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MKAA VDLK PTLT IIKT EKVD LELF PSPD MECA DVPL LTPS SKEM MSQA LKAT FSGF TKEQ QRLG IPKD PRQW TETH VRDW VMWA VNEF SLKG VDFQ KFCM NGAA LCAL GKDC FLEL APDF VGDI LWEH LEIL QKED VKPY QVNG VNPA YPES RYTS DYFI SYGI EHAQ CVPP SEFS EPSF ITES YQTL HPIS SEEL LSLK YEND YPSV ILRD PLQT DTLQ NDYF AIKQ EVVT PDNM CMGR TSRG KLGG QDSF ESIE SYDS
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IHC-P1:50 - 1:200
IF/ICC1:100 - 1:500
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:p54, ETS-1, EWSR2, c-ets-1, ETS1
Positive Sample:Mouse kidney
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:50kDa
Observed MW:52kDa

This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Purification MethodAffinity purification
Gene ID2113
RRIDAB_2763396
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Mouse kidney, using ETS1 Rabbit pAb (CAB1596) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human lung cancer using ETS1 Rabbit pAb (CAB1596) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human esophagus using ETS1 Rabbit pAb (CAB1596) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Product ImageImmunofluorescence analysis of A549 cells using ETS1 Rabbit pAb (CAB1596). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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