Description
Antibody Name: | Phospho-Cytokeratin 18 (KRT18)-S33 Antibody (CABP0263) |
Antibody SKU: | CABP0263 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting, Immunohistochemistry |
Reactivity: | Human |
Host Species: | Rabbit |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S33 of human KRT18 |
Application: | Western blotting, Immunohistochemistry |
Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 |
Reactivity: | Human |
Positive Samples: | HT-29 |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S33 of human KRT18 |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | Cytoplasm, Nucleus, nucleolus, perinuclear region |
Calculated MW: | 48kDa |
Observed MW: | 48kDa |
Synonyms: | KRT18, CK-18, CYK18, K18, keratin 18 |
Background: | KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. |
Western blot analysis of extracts from HT29 cells untreated or treated with EGF using Phospho-Cytokeratin 18 (KRT18)-S33 antibody . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. |