The Human CK-18 (KRT18) Cytokeratin 18 ELISA Kit is a reliable and accurate tool for quantifying levels of CK-18 in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.CK-18, a member of the cytokeratin family, is a key structural protein found in epithelial cells and tissues. Abnormal levels of CK-18 have been associated with various diseases and conditions, including liver diseases, cancer, and inflammatory disorders. As such, measuring CK-18 levels can provide valuable insights into disease progression, severity, and treatment efficacy.By using the Human CK-18 Cytokeratin 18 ELISA Kit, researchers can gain a better understanding of CK-18 biology and its implications in human health and disease. This kit is a valuable resource for studying CK-18-related pathways, identifying potential biomarkers, and developing novel therapeutic strategies.
Product Name:
Human CK-18/KRT18 (Cytokeratin 18) ELISA Kit
SKU:
HUES02985
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
3.75 mIU/mL
Detection range:
6.25-400 mIU/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
