Phospho-beta-Catenin-S552 Monoclonal Antibody (CABP1315)

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SKU:
CABP1315
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
Reactivity:
Human, Mouse
Synonyms:
EVR7, CTNNB, MRD19, NEDSDV, armadillo, Phospho-β-Catenin-S552
Frequently bought together:

Description

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Phospho-beta-Catenin-S552 Monoclonal Antibody (CABP1315)

The Phospho-beta-Catenin-S552 Monoclonal Antibody (CABP1315) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically detects the phosphorylated form of beta-catenin at Serine 552, a key site involved in its regulation and function.Beta-catenin is a crucial component of the Wnt signaling pathway, which has been implicated in various diseases including cancer and developmental disorders. Phosphorylation of beta-catenin at Serine 552 has been shown to affect its stability and activity, making it an important target for investigation.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.

Product Name:Phospho-beta-Catenin-S552 Monoclonal Antibody
SKU:CABP1315
Size:20μL, 100μL
Reactivity:Human, Mouse
Clone Number:ARC54001
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:RTSM G
Tested Applications:WB ELISA
Recommended Dilution:
WB1:1000 - 1:5000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:EVR7, CTNNB, MRD19, NEDSDV, armadillo, Phospho-β-Catenin-S552
Positive Sample:293T, NIH/3T3
Cellular Localization:Cell Junction, Cell Membrane, Cytoplasm, Nucleus, Adherens Junction, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle Pole.
Calculated MW:85kDa
Observed MW:92kDa

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID1499
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from 293T cells, using Phospho-β-Catenin-S552 Rabbit mAb (CABP1315) at 1:2000 dilution. 293T cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells, using Phospho-β-Catenin-S552 Rabbit mAb (CABP1315) at 1:2000 dilution. NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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