Phospho-BAD (Ser112) In-Cell ELISA

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SKU:
CBCAB01497
Product Type:
ELISA Kit
ELISA Type:
Cell Based Phospho Specific
Research Area:
Cell Death
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Detection Method:
Colorimetric

Description

Technical Manual

Phospho-BAD (Ser112) Cell-Based ELISA

Product Description:The Phospho-BAD (Ser112) Colorimetric Cell-Based ELISA Kit allows for qualitative detection of Phospho-BAD(Ser112) in plated and fixed cells.
Target Synonyms:BAD; BBC6; Bcl- XL/Bcl-2 associated death promoter; Bcl-2 binding component 6; Bcl-2-like 8 protein; Bcl-xL/Bcl-2 associated death promoter; Bcl2-antagonist of cell death; BCL2L8
Detection Target:Phospho-IRAK1(Thr209)
Reactivity:H:S75, M:S112, R:S113
Pack Size:2 x 96 assays
Detection Method:Colorimetric 450 nm
Format:Cell-Based ELISA/In-Cell ELISA
Storage and Stability Guarantee:4 °C/6 Months
Sample Type:Plated & fixed cells
Range:5000 cells/well minimum
Time:4.5 hours

Phospho-BAD (Ser112) Cell-Based ELISA Protein Information

UniProt Protein Function:BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL.
UniProt Protein Details:

Protein type:Apoptosis

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function:protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; lipid binding; protein kinase binding; protein phosphatase 2B binding

Biological Process: response to oleate; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of proteolysis; apoptosis; response to glucocorticoid stimulus; positive regulation of caspase activity; glucose homeostasis; cellular process regulating host cell cycle in response to virus; response to estradiol stimulus; positive regulation of apoptosis by virus; response to glucose stimulus; pore complex biogenesis; positive regulation of autophagy; positive regulation of glucokinase activity; caspase activation; response to drug; epidermal growth factor receptor signaling pathway; release of cytochrome c from mitochondria; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; ADP metabolic process; positive regulation of insulin secretion; response to testosterone stimulus; response to amino acid stimulus; ATP metabolic process; glucose catabolic process; response to ethanol; positive regulation of T cell differentiation; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; innate immune response; positive regulation of B cell differentiation; regulation of mitochondrial membrane permeability; response to progesterone stimulus; response to calcium ion; positive regulation of epithelial cell proliferation

NCBI Summary:The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]
UniProt Code:Q92934
NCBI GenInfo Identifier:17371773
NCBI Gene ID:572
NCBI Accession:Q92934.3
UniProt Secondary Accession:Q92934,O14803, Q6FH21,
UniProt Related Accession:Q92934
Molecular Weight:168
NCBI Full Name:Bcl2-associated agonist of cell death
NCBI Synonym Full Names:BCL2-associated agonist of cell death
NCBI Official Symbol:BAD
NCBI Official Synonym Symbols:BBC2; BCL2L8
NCBI Protein Information:bcl2-associated agonist of cell death; bcl2-L-8; BCL2-binding protein; bcl-2-like protein 8; BCL2-binding component 6; bcl-2-binding component 6; BCL-X/BCL-2 binding protein; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; bcl-XL/Bcl-2-associated death promoter
UniProt Protein Name:Bcl2-associated agonist of cell death
UniProt Synonym Protein Names:Bcl-2-binding component 6; Bcl-2-like protein 8; Bcl2-L-8; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death
Protein Family:Bcl2-associated agonist of cell death
UniProt Gene Name:BAD
UniProt Entry Name:BAD_HUMAN
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