Description
Product Name: | Phospho-AMPKa1-T183 + AMPKa2-T172 Monoclonal Antibody |
Product Code: | CABP1345 |
Reactivity: | Mouse |
Applications: | Western blotting |
Host Species: | Rabbit |
Purification Method: | Affinity purification |
Isotype: | IgG |
Clone No: | ARC53386 |
Reactivity: | Mouse |
Tested Applications: | WB, ELISA |
Key Applications: | Western blotting |
Recommended Dilution: | WB 1:500-1:1000 |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
Positive Samples: | C2C12+oligomycin |
Cellular Location: | Apical plasma membrane, Cytoplasm, Cytosol, Nuclear speck, Nucleoplasm, nucleus |
Customer Validation: | WB(Gallus gallus) |
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Immunogen: | A synthetic phosphorylated peptide around T183 of human AMPKa1 (NP_006242.5). |
Sequence: | LRTSC |
Synonyms: | AMPKa1/AMPKa2 |
Calculated MW: | 64kDa/65kDa/62kDa |
Observed MW: | 62kDa |