PDK1/PDPK1 Polyclonal Antibody (CAB21810)

(No reviews yet) Write a Review
SKU:
CAB21810
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
Reactivity:
Human, Mouse, Rat
Synonyms:
PDK1, PDPK2, PDPK2P, PRO0461, PDK1/PDPK1
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

PDK1/PDPK1 Polyclonal Antibody (CAB21810)

The PDK1/PDPK1 Polyclonal Antibody (CAB21810) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has high reactivity with human samples, making it a reliable choice for Western blot applications.PDK1/PDPK1 is a crucial enzyme that plays a pivotal role in the PI3K/Akt signaling pathway, which is often dysregulated in various diseases, including cancer, diabetes, and cardiovascular disorders. By targeting PDK1/PDPK1, researchers can gain insights into the mechanisms underlying these diseases and potentially develop new therapeutic strategies.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:PDK1/PDPK1 Polyclonal Antibody
SKU:CAB21810
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:GSNI EQYI HDLD SNSF ELDL QFSE DEKR LLLE KQAG GNPW HQFV ENNL ILKM GPVD KRKG LFAR RRQL LLTE GPHL YYVD PVNK VLKG EIPW SQEL RPEA KNFK TFFV HTPN RTYY LMDP SGNA HKWC RKIQ EVWR QRYQ SHPD AAVQ
Tested Applications:WB ELISA
Recommended Dilution:
WB1:2000 - 1:6000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:PDK1, PDPK2, PDPK2P, PRO0461, PDK1/PDPK1
Positive Sample:293T, A-549, HCT116, Rat lung
Cellular Localization:Cell Junction, Cell Membrane, Cytoplasm, Nucleus, Peripheral Membrane Protein, Focal Adhesion.
Calculated MW:63kDa
Observed MW:58-68kDa

Enables 3-phosphoinositide-dependent protein kinase activity; phospholipase activator activity; and phospholipase binding activity. Involved in several processes, including cell surface receptor signaling pathway; regulation of protein kinase activity; and regulation of signal transduction. Acts upstream of or within intracellular signal transduction. Located in cell projection; cytosol; and plasma membrane. Implicated in prostate cancer. Biomarker of lung non-small cell carcinoma.

Purification MethodAffinity purification
Gene ID5170
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using PDK1/PDPK1 Rabbit pAb (CAB21810) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
View AllClose

0 Reviews

View AllClose

Related Products