Pan Phospho-T Polyclonal Antibody (CABP1513)

Pan Phospho-T Polyclonal Antibody (CABP1513)

The Pan Phospho-T Polyclonal Antibody (CABP1513) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with phosphorylated threonine residues in proteins across various species, making it ideal for use in Western blot and immunohistochemistry applications.Phosphorylation of threonine residues is a key post-translational modification that regulates many cellular processes, including cell growth, proliferation, and differentiation. By targeting phosphorylated threonine residues in proteins, researchers can gain insights into the activation status of signaling pathways and the role of phosphorylation in disease processes.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Other (Wide Range Predicted) samples.

Western blot analysis of lysates from A-431 cells using Pan Phospho-T Rabbit pAb (CABP1513) at 1:900 dilution. A-431 cells were treated with Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.

Western blot analysis of lysates from NIH/3T3 cells using Pan Phospho-T Rabbit pAb (CABP1513) at 1:900 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.