The NSDHL Monoclonal Antibody (CAB21152) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using advanced monoclonal technology, is highly specific to human NSDHL and is validated for use in various applications such as immunofluorescence and immunohistochemistry.NSDHL, also known as NAD(P)H steroid dehydrogenase-like protein, plays a vital role in the biosynthesis of cholesterol, a key component of cell membranes and a precursor to important signaling molecules.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NSDHL Monoclonal Antibody
SKU:
CAB21152
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC3040
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
H105E3, XAP104, SDR31E1, NSDHL
Positive Sample:
HeLa, A-431
Cellular Localization:
Endoplasmic Reticulum, Lipid Droplet.
Calculated MW:
42kDa
Observed MW:
40kDa
The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene.
Purification Method
Affinity purification
Gene ID
50814
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NSDHL Rabbit mAb (CAB21152) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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