NAD/NADH Assay Kit (BA0106)

Assay Genies flurormetric NAD/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at Ex/Em = 530/585 nm, is proportional to the NAD⁺/NADH concentration in the sample. This assay is highly specific for NAD⁺/NADH with minimal interference (<1%) by NADP⁺/NADPH and is a convenient method to measure NAD, NADH and their ratio.

Applications

Direct Assays: NAD+ /NADH concentrations and ratios in cell or tissue extracts.

Key Features

• Sensitive and accurate. Detection limit of 0.02 uM and linearity up to 1 uM NAD+/NADH in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

NAD/NADH Assay Kit Information:

Kit Includes:	Assay Buffer: 10 mL Enzyme A: 120 mL Lactate: 1.5 mL Enzyme B: 120 mL Probe: 750 mL NAD Standard: 0.5 mL
Kit Requires:	Pipetting (multi-channel) devices. Black, flat bottom 96-well plates and fluorescent plate reader capable of reading at ex/em = 530/585 nm.
Method of Detection:	F530/585nm
Detection Limit:	0.02 uM
Samples:	Cell, tissue extracts etc.
Species:	All
Protocol Length:	10 min
Size:	100 tests
Storage:	Store all reagents at -20°C.
Shelf Life:	6 months