Signaling Pathway Assays
NAD/NADH Assay Kit (BA0106)
- SKU:
- BA0106
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Cell, Tissue Extracts
- Research Area:
- Glycolysis & Carbohydrates
- Research Area:
- TCA Cycle
- Research Area:
- Urea
- Research Area:
- Oxidative Stress
- Research Area:
- Antioxidant
- Research Area:
- Plant & Environmental Stress
Description
NAD/NADH Assay Kit
Pyridine nucleotidesplay an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue.
Assay Genies flurormetric NAD/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at Ex/Em = 530/585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio.
Applications
Direct Assays: NAD+ /NADH concentrations and ratios in cell or tissue extracts.Key Features
- Sensitive and accurate. Detection limit of 0.02 uM and linearity up to 1 uM NAD+/NADH in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
NAD/NADH Assay Kit Information:
| Kit Includes: | Assay Buffer: 10 mL Enzyme A: 120 mL Lactate: 1.5 mL Enzyme B: 120 mL Probe: 750 mL NAD Standard: 0.5 mL |
| Kit Requires: | Pipetting (multi-channel) devices. Black, flat bottom 96-well plates and fluorescent plate reader capable of reading at ex/em = 530/585 nm. |
| Method of Detection: | F530/585nm |
| Detection Limit: | 0.02 uM |
| Samples: | Cell, tissue extracts etc. |
| Species: | All |
| Protocol Length: | 10 min |
| Size: | 100 tests |
| Storage: | Store all reagents at -20°C. |
| Shelf Life: | 6 months |
| Smith et al. | Yeast Mannan-Rich Fraction Modulates Endogenous Reactive Oxygen Species Generation and Antibiotic Sensitivity in Resistant E. coli | Journal of Molecular Sciences 2022 | PubMed ID: 36613662 |
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