NAD-Isocitrate Dehydrogenase (NAD-IDH) Activity Assay Kit
(No reviews yet)
Write a Review
- SKU:
- MAES0246
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Animal Tissue
- Sensitivity:
- 0.84 U/L
- Range:
- 0.84-50 U/L
- Assay Time:
- 50 min
Description
| Product Name: | NAD-Isocitrate Dehydrogenase (NAD-IDH) Activity Assay Kit |
| Product Code: | MAES0246 |
| Sample Type: | Animal Tissue |
| Assay Time: | 50 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | NAD-IDH |
| Detection Instrument: | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Sensitivity: | 0.84 U/L |
| Detection Range: | 0.84-50 U/L |
| Inter-assay CV: | 0.086 |
| Intra-assay CV: | 0.05 |
Isocitrate dehydrogenase (IDH) is one of the inverters in the tricarboxylic acid cycle and plays an important role in energy metabolism, amino acid and vitamin synthesis. The cofactors of this enzyme include two kinds of NAD+ and NADP+, which are located in different parts of the cell respectively. In eukaryotic cells, NAD-dependent isocitrate dehydrogenase mainly exists in mitochondria. Under the activation of the activator, IDH converts isocitrate into α-ketoglutaric acid. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of NAD-IDH can be calculated by measuring the change of absorbance value at 450 nm.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Instruments Required: | Centrifuge,37℃ incubator |
Related Products
NAD-Malate Dehydrogenase (NAD-MDH) Activity Assay Kit
OverviewProduct Name:NAD-Malate Dehydrogenase (NAD-MDH) Activity Assay KitProduct Code:MAES0226Sample Type:Serum (Plasma), Animal Tissue, Cell
NAD+/NADH Colorimetric Assay Kit
OverviewProduct Name:NAD+/NADH Colorimetric Assay KitProduct Code:MAES0233Sample Type:Animal Tissue, CellAssay Time:70 min</
