NAD-Isocitrate Dehydrogenase (NAD-IDH) Activity Assay Kit

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SKU:
MAES0246
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Animal Tissue
Sensitivity:
0.84 U/L
Range:
0.84-50 U/L
Assay Time:
50 min

Description

Product Name:NAD-Isocitrate Dehydrogenase (NAD-IDH) Activity Assay Kit
Product Code:MAES0246
Sample Type:Animal Tissue
Assay Time:50 min
Assay Type:Enzyme Activity
Synonyms:NAD-IDH
Detection Instrument:Microplate reader (440-460 nm, optimum wavelength: 450 nm)
Sensitivity:0.84 U/L
Detection Range:0.84-50 U/L
Inter-assay CV:0.086
Intra-assay CV:0.05

Isocitrate dehydrogenase (IDH) is one of the inverters in the tricarboxylic acid cycle and plays an important role in energy metabolism, amino acid and vitamin synthesis. The cofactors of this enzyme include two kinds of NAD+ and NADP+, which are located in different parts of the cell respectively. In eukaryotic cells, NAD-dependent isocitrate dehydrogenase mainly exists in mitochondria. Under the activation of the activator, IDH converts isocitrate into α-ketoglutaric acid. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of NAD-IDH can be calculated by measuring the change of absorbance value at 450 nm.

Storage:-20°C
Valid Period:12 months
Other Instruments Required:Centrifuge,37℃ incubator
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