The Mouse TCC (C5b-9) Terminal Complement Complex ELISA Kit is a reliable and sensitive assay designed for the accurate detection of C5b-9 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity and precision, ensuring dependable and consistent results for a variety of research applications.The C5b-9 complex is a critical component of the complement system, playing a key role in immune defense and inflammation. Dysregulation of this complex has been implicated in various diseases, making it a valuable biomarker for studying immune-related disorders and developing potential therapeutic interventions.Overall, the Mouse TCC (C5b-9) Terminal Complement Complex ELISA Kit is a valuable tool for researchers looking to investigate the role of the complement system in health and disease in mouse models.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
