Mouse PARP Recombinant Protein (RPES4756)
- SKU:
- RPES4756
- Product Type:
- Recombinant Protein
- Species:
- Mouse
Description
Mouse PARP Recombinant Protein
Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
| Product Name: | Mouse PARP Recombinant Protein (RPES4756) |
| Product Code: | RPES4756 |
| Size: | 20µg |
| Species: | Mouse |
| Expressed Host: | Baculovirus-Insect Cells |
| Synonyms: | 5830444G22Rik,Adprp,Adprt1,AI893648,ARTD1,C80510,PARP,parp-1,PPOL,sPARP-1 |
| Accession: | NP_031441.2 |
| Sequence: | Met 1-Trp 1014 |
| Fusion tag: | N-His |
| Activity: | 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse PARP1 at 10 µg/mL (100 ?l/well) can bind biotinylated human HSP70, The EC50 of biotinylated human HSP70 is 0.021 µg/mL. |
| Endotoxin: | <1.0 EU per µg of the protein as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the mouse PARP1 (NP_031441.2) (Met 1-Trp 1014) was fused with a polyhistidine tag at the N-terminus. |
| Purity: | > 85 % as determined by SDS-PAGE |
| Mol Mass: | 115 kDa |
| AP Mol Mass: | 75 kDa |
| Formulation: | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly, 0.1mM TCEP |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Related Products
Human PARP Recombinant Protein (RPES4174)
Human PARP-3/PARP3 Recombinant Protein (RPES3016)
Epigenetics & Nuclear Signaling Recombinant Proteins
Mouse EGF Protein Recombinant Protein (RPPB0168)
Growth Factors & Cytokines Recombinant Proteins
Mouse TCN2 Recombinant Protein (RPES2024)
