The Mouse NF-kB p65 (Nuclear Factor kB p65) ELISA Kit is specifically designed for the accurate quantification of NF-kB p65 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.NF-kB p65 is a key transcription factor involved in regulating the expression of genes related to inflammation, immune responses, and cell survival. Dysregulation of NF-kB p65 has been linked to various diseases, including cancer, inflammatory disorders, and autoimmune conditions. Therefore, this ELISA kit is essential for studying NF-kB p65 signaling pathways and potential therapeutic interventions.Overall, the Mouse NF-kB p65 ELISA Kit from Assay Genie provides researchers with a valuable tool for investigating the role of NF-kB p65 in health and disease, offering accurate and precise measurements for insightful research discoveries.
Product Name:
Mouse NF-kB p65 (Nuclear Factor-kB p65) ELISA Kit
SKU:
MOES01305
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
