The Mouse MMP-9 (Matrix Metalloproteinase-9) CLIA Kit is specifically designed for the accurate quantification of MMP-9 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results that are essential for a wide range of research applications.MMP-9 is a key enzyme involved in tissue remodeling and extracellular matrix degradation. It plays a crucial role in various physiological processes and pathological conditions such as cancer, inflammation, and cardiovascular diseases.
By measuring MMP-9 levels, researchers can gain valuable insights into the pathological mechanisms underlying these conditions and potentially identify new therapeutic targets for intervention.Overall, the Mouse MMP-9 CLIA Kit provides researchers with a reliable and efficient tool for investigating the role of MMP-9 in disease progression and developing novel treatment strategies.
Product Name:
Mouse MMP-9 (Matrix Metalloproteinase-9) CLIA Kit
SKU:
MOES00334
Target:
Mouse MMP-9 (Matrix Metalloproteinase-9)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse MMP-9. You can calculate the concentration of Mouse MMP-9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
93-107
90-105
Average (%)
106
100
97
1:4
Range (%)
88-101
90-101
98-115
Average (%)
95
96
106
1:8
Range (%)
87-101
87-101
93-105
Average (%)
93
94
100
1:16
Range (%)
100-117
91-101
91-101
Average (%)
107
96
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
100
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
101-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.02
230.32
864.99
100.98
241.51
929.46
Standard deviation
11.25
25.98
54.84
10.31
21.23
94.15
C V (%)
11.6
11.28
6.34
10.21
8.79
10.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse MMP-9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse MMP-9 in samples. No significant cross-reactivity or interference between Mouse MMP-9 and analogues was observed.
