Mouse Cell Biology ELISA Kits 2
Mouse MMP-1 (Matrix Metalloproteinase 1) ELISA Kit (MOES01258)
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- SKU:
- MOES01258
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- Q9EPL6
- ELISA Type:
- Sandwich
- Synonyms:
- MMP1, CLG, CLGN, Interstitial Collagenase, Fibroblast Collagenase
- Reactivity:
- Mouse
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Cell Biology
- Assay Type:
- Sandwich-ELISA
- Sensitivity:
- 75 pg/mL
- Range:
- 125-8000 pg/mL
- Recovery:
- 80%-120%
Frequently bought together:
Description
Mouse MMP-1 (Matrix Metalloproteinase 1) ELISA Kit
The Mouse MMP-1 (Matrix Metalloproteinase-1) ELISA Kit is specially designed for the precise measurement of MMP-1 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, perfect for various research purposes.MMP-1 is a key enzyme involved in extracellular matrix remodeling, playing a crucial role in tissue repair, inflammation, and cancer progression. By detecting MMP-1 levels, researchers can gain valuable insights into disease processes and potential therapeutic targets, making this kit an essential tool for investigating various pathological conditions.
| Product Name: | Mouse MMP-1 (Matrix Metalloproteinase 1) ELISA Kit |
| SKU: | MOES01258 |
| Size: | 96 Assays |
| Detection Method: | Colorimetric method, ELISA, Sandwich |
| Assay type: | Sandwich-ELISA |
| Assay time: | 3 h 30 min |
| Sensitivity: | 75 pg/mL |
| Detection range: | 125-8000 pg/mL |
| Reovery: | 80%-120% |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
| Component | Specification | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells × 12 strips | 48T: 8 wells × 6 strips | 24T: 8 wells × 3 strips | 96T*5: 5 plates, 96T | -20°C, 12 months |
| Reference Standard | 96T: 2 vials | 48T/24T: 1 vial | 96T*5: 10 vials | -20°C, 12 months |
| Concentrated Biotinylated Detection Ab(100×) | 96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL | -20°C, 12 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL | -20°C (Protect from light), 12 months |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL | 2–8°C, 12 months |
| Biotinylated Detection Ab Diluent | 96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL | 2–8°C, 12 months |
| HRP Conjugate Diluent | 96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL | 2–8°C, 12 months |
| Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL | 2–8°C, 12 months |
| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL | 2–8°C (Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL | 2–8°C |
| Plate Sealer | 96T/48T/24T: 5 pieces | 96T*5: 25 pieces | 2–8°C |
| Technical Manual | 1 copy | - |
| Certificate of Analysis | 1 copy | - |
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| Uniport ID: | Q9EPL5 |
| Sample type & Sample volume: | Serum, Plasma And Other Biological Fluids; 100 μL |
| Specificity: | This kit recognizes Mouse MMP-1 in samples. No significant cross-reactivity or interference between Mouse MMP-1 and analogues was observed |
| Storage: | 2-8℃ |
| Research Area: | Cancer,Cell Biology,Cardiovascular,Signal Transduction |
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