The Mouse IL-33 ELISA Kit is specifically designed for the quantitative detection of interleukin-33 (IL-33) levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides accurate and reproducible results, making it an invaluable tool for a variety of research applications.IL-33 is a key cytokine involved in regulating immune responses and inflammation, playing a crucial role in conditions such as asthma, autoimmune diseases, and cardiovascular disorders. By accurately measuring IL-33 levels, researchers can gain valuable insights into the role of this cytokine in various diseases and explore potential therapeutic interventions.
Product Name:
Mouse IL-33 ELISA Kit
SKU:
MOES01780
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
