The Mouse IL-21 ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of interleukin 21 (IL-21) levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.IL-21 is a key cytokine involved in regulating immune responses, including the differentiation and function of various immune cells. It plays a crucial role in the development and progression of autoimmune diseases, inflammatory disorders, and cancer. By measuring IL-21 levels, researchers can gain valuable insights into the immune system and potential therapeutic targets for various diseases.Don't miss out on the opportunity to study the role of IL-21 in immune regulation and disease pathology with our Mouse IL-21 ELISA Kit. Order now and take your research to the next level.
Product Name:
Mouse IL-21 ELISA Kit
SKU:
MOES01631
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
