The Mouse IL-2 CLIA Kit is a reliable and accurate tool for detecting levels of interleukin-2 (IL-2) in mouse serum, plasma, and cell culture supernatants. This high sensitivity and specificity kit ensures precise and reproducible results, making it perfect for a variety of research applications.IL-2 is a key cytokine involved in regulating the immune response, including the proliferation and differentiation of T cells. Dysregulation of IL-2 levels has been linked to autoimmune disorders, inflammatory diseases, and cancer, making it a valuable biomarker for studying these conditions and developing therapeutic interventions.Overall, the Mouse IL-2 CLIA Kit is a valuable asset for researchers looking to study the immune system and its associated diseases in mouse models.
Product Name:
Mouse IL-2 CLIA Kit
SKU:
MOES00039
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
