The Mouse IFN-Gamma R1 (Interferon Gamma Receptor 1) ELISA Kit is a reliable and accurate testing solution for detecting levels of interferon gamma receptor 1 in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring trustworthy and repeatable results for a variety of research purposes.Interferon gamma receptor 1 plays a critical role in the immune response, specifically in the activation of macrophages and the regulation of the inflammatory process. It is involved in various immune-related disorders such as autoimmune diseases, infectious diseases, and cancer. Studying the levels of interferon gamma receptor 1 can provide valuable insights into the underlying mechanisms of these conditions and aid in the development of potential therapeutic interventions.Overall, the Mouse IFN-Gamma R1 ELISA Kit is a valuable tool for researchers seeking to explore the role of interferon gamma receptor 1 in immune responses and disease processes in mouse models.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
