Mouse Immunology ELISA Kits

Mouse Hyaluronidase ELISA Kit

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SKU:
MOFI00879
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q91ZJ9
Sensitivity:
2.344ng/ml
Range:
3.906-250ng/ml
ELISA Type:
Sandwich
Synonyms:
HAase
Reactivity:
Mouse

Description

Kit Name

Hyaluronidase is an enzyme that plays a vital role in tissue remodeling, wound healing, and various physiological processes. The Mouse Hyaluronidase ELISA Kit empowers researchers to study the dynamics of hyaluronidase levels, providing insights into its involvement in tissue homeostasis, disease models, and therapeutic interventions.

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Mouse Hyaluronidase ELISA Kit

Product Code:

MOFI00879

Size:

96 Assays

Alias:

HAase

Detection Method:

Sandwich ELISA

Application:

This immunoassay kit allows for the in vitro quantitative determination of Mouse HAase concentrations in serum plasma and other biological fluids.

Sensitivity:

2.344ng/ml

Range:

3.906-250ng/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Mouse HAase and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse HAase in samples.

88-104

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

88-98

93

EDTA plasma (n=5)

88-104

94

UFH plasma (n=5)

89-95

93

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse HAase and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

85-104%

86-103%

96-104%

EDTA plasma (n=5)

88-96%

87-100%

84-101%

UFH Plasma (n=5)

80-94%

82-90%

80-100%

Intra Assay

CV < 8%

Inter Assay

CV < 10%

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

4°C

Plate Sealer

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