The Mouse GZMS-B (Granzyme B) ELISA Kit is specifically designed for the precise measurement of granzyme B levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Granzyme B is an important enzyme that plays a crucial role in the immune response by inducing apoptosis in target cells, especially in the context of cytotoxic T lymphocytes and natural killer cells. Its dysregulation has been implicated in various diseases, including autoimmune disorders and viral infections, making it a valuable biomarker for investigating these conditions and exploring potential therapeutic interventions. The Mouse GZMS-B ELISA Kit from Assay Genie provides researchers with a reliable tool for studying the functions of granzyme B in immune responses and disease pathogenesis. Its easy-to-use format and comprehensive instructions make it a convenient choice for studies aimed at unraveling the complexities of immune-mediated diseases and developing targeted treatments.
Product Name:
Mouse Gzms-B (granzyme B) ELISA Kit
SKU:
MOES01103
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
