The Mouse GLP-1 (Glucagon-Like Peptide 1) CLIA Kit (MOES00069) is a highly sensitive and specific assay designed for the accurate detection of GLP-1 levels in mouse serum, plasma, and cell culture supernatants. This kit is essential for researchers studying metabolic disorders, diabetes, and obesity, as GLP-1 plays a key role in regulating glucose homeostasis and insulin secretion.GLP-1 is a crucial hormone involved in enhancing glucose-stimulated insulin secretion, promoting satiety, and inhibiting glucagon secretion. Dysregulation of GLP-1 levels is associated with various metabolic diseases, making it an important biomarker for understanding and developing treatments for these conditions.With its high sensitivity and reproducibility, the Mouse GLP-1 CLIA Kit is a valuable tool for researchers looking to accurately quantify GLP-1 levels in mouse samples, leading to insights into the role of this hormone in metabolic pathways and potential therapeutic interventions.
Product Name:
Mouse GLP-1 (Glucagon Like Peptide 1) CLIA Kit
SKU:
MOES00069
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
