Mouse Cell Biology ELISA Kits

Mouse FSH (follicle-stimulating hormone) ELISA Kit (MOES01033)

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SKU:
MOES01033
Product Type:
ELISA Kit
Size:
96 Assays
ELISA Type:
Sandwich
Reactivity:
Mouse
Sample Type:
Serum, plasma and other biological fluids
Assay Type:
Sandwich-ELISA
Sensitivity:
0.94 ng/mL
Range:
1.56-100 ng/mL
Recovery:
80%-120%
Frequently bought together:

Description

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Mouse FSH (follicle-stimulating hormone) ELISA Kit

The Mouse FSH (Follicle Stimulating Hormone) ELISA Kit is a powerful tool for researchers looking to accurately measure FSH levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it perfect for a variety of research applications.FSH is a key hormone involved in the regulation of the reproductive system, specifically in the development of ovarian follicles and spermatogenesis. Dysregulation of FSH levels can lead to infertility and other reproductive disorders, making it a critical biomarker for studying such conditions and potential therapeutic interventions.With the Mouse FSH ELISA Kit, researchers can confidently study the role of FSH in reproductive health and explore new avenues for treatment and intervention. Get accurate and precise results with this advanced ELISA kit from Assay Genie.

Product Name:Mouse FSH (follicle-stimulating hormone) ELISA Kit
SKU:MOES01033
Size:96 Assays
Detection Method:Colorimetric method, ELISA, Sandwich
Assay type:Sandwich-ELISA
Assay time:3 h 30 min
Sensitivity:0.94 ng/mL
Detection range:1.56-100 ng/mL
Reovery:80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
ComponentSpecificationStorage
Micro ELISA Plate (Dismountable)96T: 8 wells × 12 strips | 48T: 8 wells × 6 strips | 24T: 8 wells × 3 strips | 96T*5: 5 plates, 96T-20°C, 12 months
Reference Standard96T: 2 vials | 48T/24T: 1 vial | 96T*5: 10 vials-20°C, 12 months
Concentrated Biotinylated Detection Ab(100×)96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL-20°C, 12 months
Concentrated HRP Conjugate (100×)96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL-20°C (Protect from light), 12 months
Reference Standard & Sample Diluent96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL2–8°C, 12 months
Biotinylated Detection Ab Diluent96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL2–8°C, 12 months
HRP Conjugate Diluent96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL2–8°C, 12 months
Concentrated Wash Buffer(25×)96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL2–8°C, 12 months
Substrate Reagent96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL2–8°C (Protect from light)
Stop Solution96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL2–8°C
Plate Sealer96T/48T/24T: 5 pieces | 96T*5: 25 pieces2–8°C
Technical Manual1 copy-
Certificate of Analysis1 copy-
Linearity:
Serum (n=5)EDTA Plasma (n=5)Cell Culture Media (n=5)
1:2Range (%)90-10592-10585-95
Average (%)979990
1:4Range (%)94-10882-9197-112
Average (%)10287103
1:8Range (%)95-10986-9991-107
Average (%)1019398
1:16Range (%)94-11189-9993-109
Average (%)10194100
Recovery:
Sample TypeRange (%)Average Recovery (%)
EDTA Plasma (n=8)89-10697
Cell Culture Media (n=8)89-10496
Precision:
Intra-assay PrecisionInter-assay Precision
Sample123123
n20.020.020.020.020.020.0
Mean (ng/mL)4.6414.2545.55.0113.2549.57
Standard deviation0.220.592.670.40.844.07
C V (%)4.724.165.867.996.358.22
Uniport ID:Q60687
Sample type & Sample volume:Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:This kit recognizes Mouse FSH in samples. No significant cross-reactivity or interference between Mouse FSH and analogues was observed
Storage:2-8℃
Research Area:Cancer,Metabolism,Signal Transduction
Mallouki, B.Y., et al.Central SELENOT deficiency impairs gonadotrope axis function, sexual behavior, and fertility in male and female mice JCI Insight 2025PubMed ID: 41196650
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