Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.
Product Name:
Mouse EphB3/HEK2 Recombinant Protein (RPES2232)
Product Code:
RPES2232
Size:
100µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
AW456895,Cek10,Etk2,HEK2,MDK5,Sek4,Tyro6
Accession:
NP_034273.1
Sequence:
Met 1-Thr 537
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA. Immobilized mouse EphB3 at 2 µg/ml (100 ?l/well) can bind mouse EFNB1 with a linear range of 0.1-12.5 ng/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse EPHB3 (NP_034273.1) extracellular domain (Met 1-Thr 537) was expressed, fused with a polyhistidine tag at the C-terminus.
Purity:
> 94 % as determined by SDS-PAGE
Mol Mass:
57 kDa
AP Mol Mass:
63 kDa
Formulation:
Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
system_update_altDatasheetMouse CHK2/CHEK2 Recombinant Protein In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regula
system_update_altDatasheetMouse IFNGR2 Recombinant Protein Interferon gamma receptor beta chain (IFNgammaR2), also known as IFNGR2, belongs to the type II cytokine receptor family, whose deficien
system_update_altDatasheetMouse LAIR1 Recombinant Protein Leukocyte-associated Ig-like receptor-1 (LAIR-1) is an inhibitory receptor of the Ig superfamily that is structurally related to inhibito
system_update_altDatasheetMouse CD38 Recombinant Protein CD38, also known as ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, is a Signal-anchor for type II membrane protein. CD38 is able to tr
