Endoglin, also known as CD105, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated cytoplasmic tail. Endoglin contains an RGD tripeptide which is a key recognition structure in cellular adhesion,,suggesting a critical role for endoglin in the binding of endothelial cells to integrins and/or other RGD receptors. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, mesenchymal stem cells and leukemic cells of lymphoid and myeloid lineages. As an accessory receptor for the TGF-β superfamily ligands, endoglin binds TGF-β1 and TGF-β3 with high affinity not by itself but by associating with TGF-β type I I receptor (TβRII) and activates the downstream signal pathways. In addition, in human umbilical vein endothelial cells, ALK-1 is also a receptor kinase for endoglin threonine phosphorylation, and mutations in either of the two genes result in the autosomal-dominant vascular dysplasia, hereditary hemorrhagic telangiectasia (HHT). Endoglin has been regarded as a powerful biomarker of neovascularization, and is associated with several solid tumor types.
Product Name:
Mouse Endoglin/CD105 Recombinant Protein (RPES2790)
Product Code:
RPES2790
Size:
50µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
AI528660,AI662476,CD105,Endo,S-endoglin
Accession:
NP_031958.2
Sequence:
Met 1-Gly 580
Fusion tag:
C-His
Activity:
1. Measured by its ability to bind Human ENG-Fc in functional Elisa.2. Measured by its ability to latent TGFB1-His in functional Elisa.3. Measured by its ability to mouse ENG-His in functional Elisa.4. Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953. The ED50 for this effect is typically 5-15 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse CD105 (NP_031958.2) extracellular domain (Met 1-Gly 580) was expressed, with a C-terminal polyhistidine tag.
Purity:
> 97 % as determined by SDS-PAGE
Mol Mass:
61.2 kDa
AP Mol Mass:
65-70 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Mouse Endoglin/CD105 Recombinant Protein (His tag) (RPES5792)The product page for this antibody is being prepared. If you have a question please don't hesitate to get in touch!
system_update_altDatasheetHuman Endoglin/CD105 Recombinant Protein Endoglin, also known as CD105, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracel
system_update_altDatasheetHuman Endoglin/CD105 Recombinant Protein Endoglin, also known as CD105, is a type I homodimeric transmembrane glycoprotein with a large, disulfide-linked, extracel
system_update_altDatasheetHuman Endoglin/CD105 Recombinant Protein Endoglin is a single-pass type I membrane protein which restricted to endothelial cells in all tissues except bone marrow. Endog
