The Mouse ENA-78 (Epithelial Neutrophil Activating Peptide-78) ELISA Kit is a reliable and accurate tool for the quantitative detection of ENA-78 levels in mouse serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring precise and consistent results for a variety of research applications.ENA-78, also known as C-X-C motif chemokine 5 (CXCL5), is a key chemokine involved in the recruitment and activation of neutrophils, contributing to inflammatory responses. Elevated levels of ENA-78 have been implicated in various inflammatory conditions and diseases, making it a valuable biomarker for studying inflammation and developing potential therapeutic interventions.With its user-friendly protocol and high-quality components, the Mouse ENA-78 ELISA Kit from Assay Genie is a valuable tool for researchers investigating the role of ENA-78 in inflammation, immune responses, and disease pathogenesis. Order yours today and advance your research with confidence.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
