The Mouse Col2 (Collagen Type 2) CLIA Kit is specifically designed for the quantitative determination of collagen type 2 levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Collagen type 2 is a key component of cartilage and plays a critical role in maintaining the structural integrity of joints. Changes in collagen type 2 levels have been linked to conditions such as osteoarthritis and rheumatoid arthritis, making it a valuable marker for studying these diseases and developing potential therapeutic interventions. With the Mouse Col2 (Collagen Type 2) CLIA Kit, researchers can confidently measure collagen type 2 levels in mouse samples, providing valuable insights into the pathogenesis of joint-related disorders and facilitating the development of novel treatment strategies.
Product Name:
Mouse COL2 (Collagen Type 2) CLIA Kit
SKU:
MOES00210
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
