In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by CHEK2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded CHEK2 protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in CHEK2s gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Product Name:
Mouse CHK2/CHEK2 Recombinant Protein (RPES1974)
Product Code:
RPES1974
Size:
20µg
Species:
Mouse
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
Cds1,CHK2,HUCDS1,Rad53
Accession:
Q9Z265
Sequence:
Mey 1-Leu 546
Fusion tag:
N-His-GST
Activity:
Kinase activity untested
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse CHEK2 (Q9Z265) (Mey 1-Leu 546) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Purity:
> 90 % as determined by SDS-PAGE
Mol Mass:
89 kDa
AP Mol Mass:
90 kDa
Formulation:
Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
system_update_altDatasheetHuman CHK2/CHEK2 Recombinant Protein In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regula
system_update_altDatasheetMouse FSTL1 Recombinant Protein Follistatin-like 1 (FSTL1) is a secreted glycoprotein that has been grouped into the follistatin family of proteins. FSTL1 is composed of
system_update_altDatasheetMouse SLAMF8 Recombinant Protein The signaling lymphocyte activation molecule (SLAM) family includes homophilic and heterophilic receptors that modulate both adaptive
system_update_altDatasheetMouse AKT3 Recombinant Protein v-akt murine thymoma viral oncogene homolog 3 (AKT3), also known as PKB-GAMMA, with AKT1/PKBalpha, AKT2/PKBbeta, are the memerbers of Akt
