The Mouse CFB (Complement Factor B) ELISA Kit is specifically designed for the precise measurement of complement factor B levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results, making it perfect for various research purposes.Complement factor B is a key component in the complement system, playing a vital role in immune response and inflammation. Dysregulation of complement factor B has been linked to various diseases, including autoimmune disorders and inflammatory conditions, making it a valuable biomarker for studying these diseases and exploring potential treatments.Overall, the Mouse CFB ELISA Kit from Assay Genie provides researchers with a reliable and efficient tool for investigating the role of complement factor B in biological processes and disease pathogenesis.
Product Name:
Mouse CFB (Complement Factor B) ELISA Kit
SKU:
MOES00897
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
