Mouse Cell Signalling ELISA Kits 5
Mouse CD62P/P-selectin ELISA Kit (MOFI00087)
- SKU:
- MOFI00087
- Product Type:
- ELISA Kit
- Uniprot:
- Q01102
- ELISA Type:
- Sandwich
- Synonyms:
- P-Selectin, CD62P, GMP140, GRMP, PADGEM, PSEL, SELP, CD62P antigen, FLJ45155, PADGEMantigen CD62, selectin P
- Reactivity:
- Mouse
- Size:
- 48T/96T
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 9.375 pg/mL
- Range:
- 15.625-1000 pg/mL
Description
Mouse CD62P/P-selectin ELISA Kit
The Mouse CD62P (P-Selectin) ELISA Kit is a powerful tool for researchers studying inflammation, thrombosis, and immune response in mouse models. This kit allows for the sensitive and accurate detection of CD62P levels in mouse serum, plasma, and cell culture supernatants, providing researchers with valuable insights into the biology of P-selectin. CD62P is a cell adhesion molecule that is crucial for the recruitment and activation of immune cells at sites of inflammation and injury. It plays a pivotal role in the inflammatory response and thrombus formation, making it a key target for studying various diseases, including atherosclerosis, stroke, and autoimmune disorders.
With its high sensitivity and specificity, the Mouse CD62P (P-Selectin) ELISA Kit offers reliable and reproducible results, making it an essential tool for research in immunology, cardiovascular biology, and other related fields. By accurately measuring CD62P levels, researchers can gain a better understanding of the underlying mechanisms of disease and work towards developing novel therapeutic strategies.
| Product Name: | Mouse CD62P/P-selectin ELISA Kit |
| SKU: | MOFI00087 |
| Reactivity: | Mouse |
| Assay Type: | Sandwich ELISA, Double Antibody |
| Sensitivity: | 9.375 pg/mL |
| Range: | 15.625-1000 pg/mL |
| Sample Type: | Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples |
| Storage: | 2-8°C for 12 months. |
| Linearity: |
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| Recovery: |
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Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions. |
| 2 | Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding. |
| 3 | Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes. |
| 4 | HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes. |
| 5 | Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes. |
| 6 | Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately. |
| Sample Type | Protocol |
| Serum | Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately. |
| Plasma | Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma. |
| Tissue Homogenate | Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant. |
| Cell Culture Supernatant | Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant. |
| Cell Lysate | Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant. |
| Other Sample Types | For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com. |
| Component | Quantity | Storage | |
| 48T | 96T | ||
| ELISA Microplate (Dismountable) | 8×6 | 8×12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Lyophilized Standard | 1 vial | 2 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Biotin-labeled Antibody (Concentrated, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| HRP-Streptavidin Conjugate (SABC, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| TMB Substrate | 5 ml | 10 ml | 2-8°C (Avoid direct light) |
| Sample Dilution Buffer | 10 ml | 20 ml | 2-8°C |
| Antibody Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| SABC Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| Stop Solution | 5 ml | 10 ml | 2-8°C |
| Wash Buffer(25X) | 15 ml | 30 ml | 2-8°C |
| Plate Sealer | 3 pieces | 5 pieces | - |
| Technical Manual | 1 copy | 1 copy | - |
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