Mouse CD45R/B220 Monoclonal Antibody (Alexa Fluor 488 Conjugated) [RA3.3A 1/6.1](AGEL0425)

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SKU:
AGEL0425
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Host Species:
Rat
Isotype:
IgM
Clone:
RA3.3A 1/6.1
Conjugation:
AF 488
Applications:
FC

Description

system_update_altDatasheet
Antibody Name:Mouse CD45R/B220 Monoclonal Antibody (Alexa Fluor 488 Conjugated)
Antibody Code:AGEL0425
Size:50 Tests
Clone:RA3.3A 1/6.1
Synonyms:B220, CD45, GP180, LCA, T200
Host Species:Rat
Species Reactivity:Mouse
Applications:FC
Concentration:5uL
Isotype:IgM
Conjugation:Alexa Fluor 488
Applications:FC
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 uL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:PBS with 0.05% Proclin300, 1% BSA
Storage:Keep as concentrated solution.Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Gene ID:192645788
Uniprot:P06800, P08575
Background:CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-2CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is eessed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.40 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. s.
Mouse CD19 Monoclonal Antibody (FITC Conjugated)C57BL/6 murine splenocytes are stained with Anti-Mouse CD45R/B220 Monoclonal Antibody (Alexa Fluor 488 Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.
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