Description
Antibody Name: | Mouse CD45.2 Monoclonal Antibody (FITC Conjugated) |
Antibody Code: | AGEL1515 |
Size: | 200 Tests |
Clone: | 104.2 |
Synonyms: | Ly-5.2, LCA |
Host Species: | Mouse |
Species Reactivity: | Mouse |
Applications: | FC |
Concentration: | 5uL |
Isotype: | IgG2a,k |
Conjugation: | FITC |
Applications: | FC |
Recommended Dilution: | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 uL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
Storage Buffer: | PBS with 0.05% Proclin300, 1% BSA |
Storage: | Store at 2-8°C and protected from prolonged exposure to light. Do not freeze. |
Gene ID: | 19264 |
Uniprot: | P06800 |
Background: | CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1. |
C57BL/6 murine splenocytes are stained with Anti-Mouse CD45.2 Monoclonal Antibody(FITC Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control. |