Mouse CD45.2 Monoclonal Antibody (AF647 Conjugated) [104.2](AGEL1534)

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SKU:
AGEL1534
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FC
Reactivity:
Mouse
Host Species:
Mouse
Isotype:
IgG2a,k
Clone:
104.2
Conjugation:
AF 647

Description

Antibody Name:Mouse CD45.2 Monoclonal Antibody (AF647 Conjugated)
Antibody Code:AGEL1534
Size:50µg
Clone:104.2
Synonyms:Ly-5.2, LCA
Host Species:Mouse
Species Reactivity:Mouse
Applications:FC
Concentration:1mg/mL
Isotype:IgG2a,k
Conjugation:AF647
Applications:FC
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer:PBS with 0.05% Proclin300, 1% BSA
Storage:Store at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Gene ID:19264
Uniprot:P06800
Background:CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Mouse CD19 Monoclonal Antibody (FITC Conjugated)C57BL/6 murine splenocytes are stained with Anti-Mouse CD45.2 Monoclonal Antibody(AF647 Conjugated)[Used at ?g/106 cells dilution](filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.
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