Mouse CD162 Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated) [4RA10](AGEL0845)

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SKU:
AGEL0845
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FC
Reactivity:
Mouse
Host Species:
Rat
Isotype:
IgG2a,k
Clone:
4RA10
Conjugation:
PerCP/Cy5.5

Description

Antibody Name:Mouse CD162 Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated)
Antibody Code:AGEL0845
Size:50 Tests
Clone:4RA10
Synonyms:P-selectin glycoprotein ligand 1,Selplg,PSGL-1,Selectin P ligand,CD162
Host Species:Rat
Species Reactivity:Mouse
Applications:FC
Concentration:5uL
Isotype:IgG2a,k
Conjugation:PerCP/Cyanine5.5
Applications:FC
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 uL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage Buffer:PBS with 0.05% Proclin300, 1% BSA
Storage:Store at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Gene ID:20345
Uniprot:Q62170
Background:The 4RA10 antibody reacts with the N-terminal functional peptide of CD162 (P-selectin glycoprotein ligand-1, PSGL-1), encoded by the Selpl gene. PSGL-1 is expressed on the cell surface as a homodimer of approximately 230 kDa. In the mouse, Selpl mRNA is detected in most tissues, with high levels found in hematopoietic cells, brain, and adipose tissue. Flow cytometric analyses have revealed CD162 expression on bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and neutrophil and T-cell lines. PSGL-1 is a ligand for P-selectin (CD62P) and is involved in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury. It is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. There is also evidence that other ligands for PSGL-1 and CD62P may exist. 4RA10 mAb is reported to block the binding of mouse leukocytes to CD62P and CD62L.
Mouse CD19 Monoclonal Antibody (FITC Conjugated)C57BL/6 murine splenocytes are stained with Anti-Mouse CD162 Monoclonal Antibody(PerCP/Cyanine5.5 Conjugated)(filled gray histogram). Unstained splenocytes (empty black histogram) are used as control.
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