Mouse CD103 Monoclonal Antibody (AF647 Conjugated) [M290](AGEL1162)

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SKU:
AGEL1162
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Applications:
FC
Reactivity:
Mouse
Host Species:
Rat
Isotype:
IgG2a,k
Clone:
M290
Conjugation:
AF 647

Description

Antibody Name:Mouse CD103 Monoclonal Antibody (AF647 Conjugated)
Antibody Code:AGEL1162
Size:50µg
Clone:M290
Synonyms:Integrin alpha-E,Itgae,Integrin alpha M290,CD103
Host Species:Rat
Species Reactivity:Mouse
Applications:FC
Concentration:1mg/mL
Isotype:IgG2a,k
Conjugation:AF647
Applications:FC
Recommended Dilution:Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10e6 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Storage Buffer:PBS with 0.05% Proclin300, 1% BSA
Storage:Store at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Gene ID:16407
Uniprot:Q60677
Background:CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin αIEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.
Mouse CD19 Monoclonal Antibody (FITC Conjugated)C57BL/6 murine splenocytes are stained with Anti-Mouse CD103 Monoclonal Antibody(AF647 Conjuaged) and Anti-Mouse CD3 Monoclonal Antibody(AF488 Conjugated)[Both antibodies are used at 0.2 ?g/106 cells dilution]. Splenocytes stained with Anti-Mouse CD3 Monoclonal Antibody(AF488 Conjugated) (right) are used as control.
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