Mouse beta-Hex A (beta-hexosaminidase A) ELISA Kit
The Mouse Beta-Hex A/Beta-Hexosaminidase A ELISA Kit is specifically designed for the accurate detection of beta-hexosaminidase A levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Beta-hexosaminidase A is an enzyme involved in lysosomal metabolism, playing a critical role in the breakdown of glycosphingolipids. Dysregulation of this enzyme has been linked to various lysosomal storage disorders, making it a valuable biomarker for studying these conditions and developing potential treatments. With this ELISA kit, researchers can easily quantify beta-hexosaminidase A levels in mouse samples, providing valuable insights into lysosomal function and potential therapeutic targets. Trust in the accuracy and precision of the Mouse Beta-Hex A/Beta-Hexosaminidase A ELISA Kit for your research needs.
Product Name:
Mouse beta-Hex A (beta-hexosaminidase A) ELISA Kit
SKU:
MOES01626
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
