Mouse Cell Signalling ELISA Kits 1

Mouse ALT ELISA Kit

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SKU:
MOFI00634
Product Type:
ELISA Kit
Size:
96 Assays
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
ELISA Type:
Sandwich
Synonyms:
ALT, Alanine Transaminase, ALAT, SGPT, alanine aminotransferase, serum glutamic pyruvic transaminase
Reactivity:
Mouse

Description

Mouse ALT ELISA Kit

Intro paragraph

system_update_alt Datasheet system_update_alt MSDS

Key Features

Save Time Pre-coated 96 well plate
Quick Start Kit includes all necessary reagents
Publication Ready Reproducible and reliable results

Overview

Product Name:

Mouse ALT ELISA Kit

Product Code:

MOFI00634

Size:

96 Assays

Alias:

ALT, Alanine Transaminase, ALAT, SGPT, alanine aminotransferase, serum glutamic pyruvic transaminase

Detection Method:

Sandwich ELISA

Application:

This immunoassay kit allows for the in vitro quantitative determination of Mouse ALT concentrations in serum plasma and other biological fluids.

Sensitivity:

0.094ng/ml

Range:

0.156-10ng/ml

Storage:

4°C for 6 months

Note:

For Research Use Only

Additional Information

Recovery

Matrices listed below were spiked with certain level of Mouse ALT and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse ALT in samples.

Matrix

Recovery Range (%)

Average (%)

serum (n=5)

93-105

99

EDTA plasma (n=5)

91-101

95

UFH plasma (n=5)

87-102

95

Linearity:

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse ALT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

Serum (n=5)

87-100%

85-101%

89-99%

EDTA plasma (n=5)

83-100%

85-100%

82-91%

UFH Plasma (n=5)

92-96%

83-89%

83-100%

CV(%)

Intra Assay <8

Inter Assay <10

Kit Components

Component Quantity Storage

ELISA Microplate (Dismountable)

8x12 strips

4°C for 6 months

Lyophilized Standard

2

4°C/ -20°C

Sample/Standard Dlution Buffer

20ml

4°C

Biotin-labeled Antibody (Concentrated)

120ul

4°C (Protection from light)

Antibody Dilution Buffer

10ml

4°C

HRP-Streptavidin Conjugate (SABC)

120ul

4°C (Protect from light)

SABC Dilution Buffer

10ml

4°C

TMB Substrate

10ml

4°C (Protection from light)

Stop Solution

10ml

4°C

Wash Buffer (25X)

30ml

4°C

Plate Sealer

5

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