Monkey CD30L (Tumor necrosis factor ligand superfamily member 8) ELISA Kit (MKFI0118)
- SKU:
- MKFI0118
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Reactivity:
- Monkey
- Size:
- 48T/96T
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 4.688 pg/mL
- Range:
- 7.813-500 pg/mL
Description
Monkey CD30L (Tumor necrosis factor ligand superfamily member 8) ELISA Kit
The Monkey CD30L (Tumor Necrosis Factor Ligand Superfamily Member 8) ELISA Kit is a highly specific and sensitive assay designed for the precise detection of CD30L levels in monkey serum, plasma, and cell culture supernatants. This kit is perfect for researchers looking to investigate the role of CD30L in various biological processes. CD30L is a key member of the tumor necrosis factor ligand superfamily, playing a crucial role in immune responses and cell death pathways. Dysregulation of CD30L has been implicated in various diseases, including cancer and autoimmune disorders, making it a valuable target for therapeutic intervention.
With its reliable performance and ease of use, the Monkey CD30L ELISA Kit is an essential tool for studying the function of CD30L and its potential implications in disease progression. Get accurate and reproducible results with this kit, allowing you to advance your research and uncover new insights into the role of CD30L in monkey biology.
| Product Name: | Monkey CD30L (Tumor necrosis factor ligand superfamily member 8) ELISA Kit |
| SKU: | MKFI0118 |
| Reactivity: | Monkey |
| Assay Type: | Sandwich ELISA, Double Antibody |
| Sensitivity: | 4.688 pg/mL |
| Range: | 7.813-500 pg/mL |
| Sample Type: | Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples |
| Storage: | 2-8°C for 12 months. |
| Linearity: |
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| Recovery: |
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Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions. |
| 2 | Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding. |
| 3 | Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes. |
| 4 | HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes. |
| 5 | Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes. |
| 6 | Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately. |
| Sample Type | Protocol |
| Serum | Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately. |
| Plasma | Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma. |
| Tissue Homogenate | Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant. |
| Cell Culture Supernatant | Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant. |
| Cell Lysate | Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant. |
| Other Sample Types | For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com. |
| Component | Quantity | Storage | |
| 48T | 96T | ||
| ELISA Microplate (Dismountable) | 8×6 | 8×12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Lyophilized Standard | 1 vial | 2 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Biotin-labeled Antibody (Concentrated, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| HRP-Streptavidin Conjugate (SABC, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| TMB Substrate | 5 ml | 10 ml | 2-8°C (Avoid direct light) |
| Sample Dilution Buffer | 10 ml | 20 ml | 2-8°C |
| Antibody Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| SABC Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| Stop Solution | 5 ml | 10 ml | 2-8°C |
| Wash Buffer(25X) | 15 ml | 30 ml | 2-8°C |
| Plate Sealer | 3 pieces | 5 pieces | - |
| Technical Manual | 1 copy | 1 copy | - |
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