Monkey ADAMTS13 (A disintegrin and metalloproteinase with thrombospondin motifs 13) ELISA Kit (AEFI00510)

Monkey ADAMTS13 (A disintegrin and metalloproteinase with thrombospondin motifs 13) ELISA Kit

The Monkey ADAMTS13 (A Disintegrin and Metalloproteinase with Thrombospondin Motifs 13) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of ADAMTS13 levels in monkey serum, plasma, and tissue culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for research applications related to ADAMTS13 function and regulation. ADAMTS13 is a key enzyme involved in the regulation of blood clot formation and breakdown, specifically targeting von Willebrand factor. Dysregulation of ADAMTS13 activity has been implicated in diseases such as thrombotic thrombocytopenic purpura (TTP) and other thrombotic disorders, highlighting the importance of studying its levels and activity in various disease models.

By utilizing the Monkey ADAMTS13 ELISA Kit, researchers can gain valuable insights into the role of ADAMTS13 in health and disease, paving the way for potential therapeutic interventions targeting this critical enzyme. Whether investigating the pathophysiology of thrombotic disorders or exploring new treatment approaches, this kit provides a valuable tool for advancing our understanding of ADAMTS13 biology.

Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Step	Procedure
1	Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2	Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3	Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4	HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5	Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6	Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.