MOB1A Antibody (PACO41102)

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SKU:
PACO41102
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
Applications:
WB
Applications:
IHC
Applications:
IF
Applications:
IP
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:MOB1A Antibody (PACO41102)
Antibody SKU:PACO41102
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, WB, IHC, IF, IP
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:2000-1:5000, IHC:1:100-1:1000, IF:1:200-1:500, IP1:200-1:2000,
Species Reactivity:Human
Immunogen:Recombinant Human MOB kinase activator 1A protein (2-216AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageImmunohistochemistry of paraffin-embedded human small intestine tissue using PACO41102 at dilution of 1:100.
Product ImageIHC image of PACO41102 diluted at 1:1000 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product ImageWestern Blot. Positive WB detected in: K562 whole cell lysate, Jurkat whole cell lysate. All lanes: MOB1A antibody at 3.3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 26, 17 kDa. Observed band size: 26 kDa.
Background:Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38.
Synonyms:MOB kinase activator 1A (Mob1 alpha) (Mob1A) (Mob1 homolog 1B) (Mps one binder kinase activator-like 1B), MOB1A, C2orf6 MOB4B MOBK1B MOBKL1B
UniProt Protein Function:MOB1A: Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38. Binds STK38 and STK38L. Interacts with LATS1 and LATS2. Forms a tripartite complex with STK38 and STK3/MST2. Adrenal gland, bone marrow, brain, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, heart, spinal cord, fetal brain and fetal liver. Belongs to the MOB1/phocein family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: cytosol

Molecular Function:protein binding; metal ion binding

NCBI Summary:The protein encoded by this gene is a component of the Hippo signaling pathway, which controls organ size and tumor growth by enhancing apoptosis. Loss of the encoded protein results in cell proliferation and cancer formation. The encoded protein is also involved in the control of microtubule stability during cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
UniProt Code:Q9H8S9
NCBI GenInfo Identifier:56749356
NCBI Gene ID:55233
NCBI Accession:Q9H8S9.4
UniProt Related Accession:Q9H8S9
Molecular Weight:
NCBI Full Name:MOB kinase activator 1A
NCBI Synonym Full Names:MOB kinase activator 1A
NCBI Official Symbol:MOB1A  
NCBI Official Synonym Symbols:MOB1; MATS1; Mob4B; C2orf6; MOBK1B; MOBKL1B  
NCBI Protein Information:MOB kinase activator 1A
UniProt Protein Name:MOB kinase activator 1A
UniProt Synonym Protein Names:Mob1 alpha; Mob1A; Mob1 homolog 1B; Mps one binder kinase activator-like 1B
Protein Family:MOB kinase activator
UniProt Gene Name:MOB1A  
UniProt Entry Name:MOB1A_HUMAN
Antibodies
MOB1A Antibody (PACO10536)
Secondary Antibody
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