Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit
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- SKU:
- MAES0224
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Animal Tissue
- Sensitivity:
- 3.0 U/L
- Range:
- 3.0-88.6 U/L
- Assay Time:
- 10 min
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Description
| Product Name: | Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit |
| Product Code: | MAES0224 |
| Sample Type: | Animal Tissue |
| Assay Time: | 10 min |
| Assay Type: | Enzyme Activity |
| Detection Instrument: | Microplate reader (540-560 nm, optimum wavelength: 550 nm) |
| Detection Method: | Colorimetric method |
| Sensitivity: | 3.0 U/L |
| Detection Range: | 3.0–88.6 U/L |
| Inter-assay CV: | 0.095 |
| Intra-assay CV: | 0.05 |
Mitochondrial complex IV can catalyze the oxidation of reduced cytochrome C to oxidized cytochrome C, which has an absorption wavelength at 550 nm. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 550 nm.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Reagents Required: | Anhydrous ethanol, PBS(0.01 M，pH 7.4) |
| Other Instruments Required: | Centrifuge |
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