Mitochondrial Complex II Activity Assay Kit

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SKU:
MAES0223
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Animal Tissue, Cell
Sensitivity:
0.54 U/L
Range:
0.54-17.66 U/L
Assay Time:
15 min

Description

Product Name:Mitochondrial Complex II Activity Assay Kit
Product Code:MAES0223
Sample Type:Animal Tissue, Cell
Assay Time:15 min
Assay Type:Enzyme Activity
Detection Instrument:Microplate reader (590-610 nm, optimum wavelength: 600 nm)
Detection Method:Colorimetric method
Sensitivity:0.54 U/L
Detection Range:0.54-17.66 U/L
Inter-assay CV:0.05
Intra-assay CV:0.04

Coenzyme Q, the catalytic product of mitochondrial complex II, can further reduce 2, 6-dichloroindoxol, which has a characteristic absorption peak at 600 nm. Therefore, the activity of mitochondrial complex II can be quantified by measure the change OD value at 600 nm.

Storage:2-8°C
Valid Period:12 months
Other Reagents Required:Normal saline (0.9% NaCl), PBS(0.01 M，pH 7.4)
Other Instruments Required:Centrifuge
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