Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit

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SKU:
MAES0240
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Animal Tissue
Sensitivity:
15.58 U/L
Range:
15.58-224.2 U/L
Assay Time:
15 min

Description

Product Name:Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit
Product Code:MAES0240
Sample Type:Animal Tissue
Assay Time:15 min
Assay Type:Enzyme Activity
Detection Instrument:Microplate reader (340 nm)
Detection Method:Colorimetric method
Sensitivity:15.58 U/L
Detection Range:15.58-224.2 U/L
Inter-assay CV:0.084
Intra-assay CV:0.008

Mitochondrial complex I catalyzes the reaction of NADH with ubiquinone substrate to generate NAD+ and reduced ubiquinone. The specific inhibitor rotenone can inhibit the enzyme, and the total enzyme activity minus the enzyme activity after inhibitor inhibition can reflect its specific enzyme activity.

Storage:-20°C
Valid Period:12 months
Other Reagents Required:Anhydrous ethanol (AR)
Other Instruments Required:Centrifuge, 37℃ incubator
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