Metabolism Assays

LDH assay kit / Lactate Dehydrogenase Assay kit (Colorimetric) (BA0006)

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SKU:: BA0006
UPC:
MPN:
Product Type:: Assay
Detection Method:: Colorimetric
Instrument:: Microplate Reader
Sample Type:: Serum, Plasma
Research Area:: Liver Biomarker
Research Area:: Plant & Environmental Stress
Research Area:: Enzyme Activity
Research Area:: Clinical Chemistry

Description
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Description

Lactate Dehydrogenase Assay - Information

LACTATE DEHYDROGENASE(LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. When disease or injury affects tissues containing LDH, the cells release LDH into the bloodstream, where it is identified in higher than normal levels. Therefore, LDH is most often measured to evaluate the presence of tissue or cell damage. The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the enzyme activity.

Applications

For quantitative determination of lactate dehydrogenase LDH activity and screen/evaluation of LDH modulators.

Lactate Dehydrogenase Assay - Key Features

High sensitivity and wide linear range. Use 3 serum or plasma sample. The detection limit is 2 U/L, linear up to 200 U/L
Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of LDH activity within 30 minutes.
Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.

Lactate Dehydrogenase Assay - Data Sheet
Method of Detection	OD565nm
Detection Limit	2 U/L
Samples	Serum, plasma etc
Species	All
Protocol Length	30 min
Size	100 tests
Shelf Life	6 months