Name: Lcn2 Antibody (PACO64291)
SKU: PACO64291
Availability: OutOfStock

Antibody Name:	Lcn2 Antibody (PACO64291)
Antibody SKU:	PACO64291
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	IHC:1:100-1:500
Species Reactivity:	Rat
Immunogen:	Recombinant Rat Lcn2 protein (1-198AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	Antigen Affinity Purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO64291 diluted at 1:200 and staining in paraffin-embedded rat lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Background:

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development (By similarity). Binds iron through association with 2,5-dihydroxybenzoic acid, (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis (By similarity). Involved in innate immunity; limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis (By similarity).

Synonyms:

Neutrophil gelatinase-associated lipocalin (NGAL) (Alpha-2-microglobulin-related protein) (Alpha-2U globulin-related protein) (Lipocalin-2) (Siderocalin LCN2) (p25), Lcn2

UniProt Protein Function:	LCN2: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. Belongs to the calycin superfamily. Lipocalin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transporter; Apoptosis; Secreted, signal peptide; Secreted Cellular Component: extracellular space; cell; extracellular region; cytosol Molecular Function:protein homodimerization activity; protease binding; transporter activity; iron ion binding Biological Process: response to drug; response to nutrient levels; response to mycotoxin; response to virus; positive regulation of cell projection organization and biogenesis; response to herbicide; cellular response to nutrient levels; iron ion homeostasis; response to bacterium; siderophore transport; innate immune response; ion transport; response to oxidative stress
NCBI Summary:	mouse homolog plays a role in IL3 withdrawal-induced apoptosis [RGD, Feb 2006]
UniProt Code:	P30152
NCBI GenInfo Identifier:	306526224
NCBI Gene ID:	170496
NCBI Accession:	P30152.2
UniProt Secondary Accession:	P30152,Q5HZF1,
UniProt Related Accession:	P30152
Molecular Weight:	22,476 Da
NCBI Full Name:	Neutrophil gelatinase-associated lipocalin
NCBI Synonym Full Names:	lipocalin 2
NCBI Official Symbol:	Lcn2
NCBI Official Synonym Symbols:	Sip24
NCBI Protein Information:	neutrophil gelatinase-associated lipocalin; p25; NGAL; lipocalin-2; siderocalin LCN2; lipocalin 2 (oncogene 24p3); alpha-2U globulin-related protein; alpha-2-microglobulin-related protein
UniProt Protein Name:	Neutrophil gelatinase-associated lipocalin
UniProt Synonym Protein Names:	Alpha-2-microglobulin-related protein; Alpha-2U globulin-related protein; Lipocalin-2; Siderocalin LCN2; p25
Protein Family:	Neutrophil gelatinase-associated lipocalin
UniProt Gene Name:	Lcn2
UniProt Entry Name:	NGAL_RAT