Signaling Pathway Assays
L-Glutamate Quick Test Strips (BA0198)
- SKU:
- BA0198
- Product Type:
- Assay
- Sample Type:
- Soups, Sauces, Milk
- Research Area:
- TCA Cycle
- Research Area:
- Food Safety & Analysis
Description
L-Glutamate Quick Test Strips (BA0198) (BA0198)
The L-Glutamate Quick Test Strips (SKU: BA0198) offer a rapid, instrument-free method for estimating glutamate concentrations in a range of food samples. Glutamate is an important metabolite that is also widely used as a flavour enhancer in the food industry, and consumption of foods containing monosodium glutamate (MSG) has been associated with reactions such as headaches, flushing, sweating and nausea. It is also a crucial mammalian neurotransmitter implicated in a number of neurological and psychiatric disorders. These strips are based on glutamate dehydrogenase-catalysed oxidation of glutamate, in which the NADH formed reduces a chromogenic reagent to produce a colour proportional to the glutamate concentration. The developed reaction pad is simply compared against a reference chart and multiplied by the dilution factor to obtain the result, with the whole procedure completed in around fifteen minutes.
| Product Name: | L-Glutamate Quick Test Strips (BA0198) |
| SKU: | BA0198 |
| Detection Method: | Visual (colorimetric test strip). Based on glutamate dehydrogenase-catalysed oxidation of glutamate, in which the NADH formed reduces a chromogenic reagent. The intensity of the product colour is directly proportional to the glutamate concentration in the sample and is read by comparison against a reference chart. |
| Detection Range: | Semi-quantitative measurement of L-glutamate in the sample |
| Sample Type: | Food samples such as instant soups, acidic samples, soy sauces, fish sauces and milk |
| Species Reactivity: | All |
| Assay Time: | Approximately 15 minutes |
| Kit Size: | 10 tests |
| Equipment Required: | Benchtop |
| Storage: | Store at room temperature. Keep strips dry and out of direct sunlight. |
| Shelf Life: | 6 months upon receipt |
| Shipping: | Room Temperature |
These test strips estimate L-glutamate semi-quantitatively using glutamate dehydrogenase-catalysed oxidation of glutamate, in which the NADH formed reduces a chromogenic reagent. After a sample is developed in the supplied development tube, a strip is dipped, colour is allowed to develop for five minutes and the reaction pad is compared against the provided glutamate chart. The reading is multiplied by the dilution factor (e.g. 5x, 21x or 210x) to obtain the glutamate concentration in the original sample.
- Fast and convenient. Results in approximately 15 minutes.
- No expensive laboratory equipment required.
- Suitable for a range of food samples including soups, sauces and milk.
- Direct determination of L-glutamate concentrations in food samples such as instant soups, soy sauces, fish sauces and milk.
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Unscrew the cap of one of the Sample Development tubes. |
| 2 | For samples requiring a 21x dilution, transfer 20 µL of sample into the Sample Development Tube using a 20 µL transfer pipette or pipettor. For samples requiring a 5x dilution, transfer 100 µL of sample using a 100 µL transfer pipette or pipettor. For samples requiring a 210x dilution, first prepare a 10x dilution by transferring 100 µL of sample into a Sample Dilution Tube containing 900 µL of distilled water, mix, then transfer 20 µL of this diluted sample into a Sample Development Tube. |
| 3 | Replace the cap, close securely and invert the vial a couple of times to mix. |
| 4 | Unscrew the Sample Development Tube cap and dip in one of the test strips, fully submerging the reaction pad. Leave submerged for 5 seconds, then remove and shake to remove any clinging drops. |
| 5 | Let the colour develop on the strip for 5 minutes. |
| 6 | Compare the colour of the reaction pad with the provided Glutamate Chart on the test strip bag and multiply the concentration by the dilution used (i.e. 5x, 21x or 210x). |
Read the glutamate concentration by comparing the developed reaction pad against the provided Glutamate Chart, then multiply the chart value by the dilution factor used (5x, 21x or 210x) to obtain the glutamate concentration in the sample.
| Component | Quantity | Storage |
| Test Strips | 10 | Room Temperature |
| Sample Development Tubes (400 µL Development Reagent per tube) | 10 | Room Temperature |
| Instruction Manual | 1 | Room Temperature |
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